Journal: eBioMedicine

Article Title: Pharmacological inhibition of tyrosine protein-kinase 2 reduces islet inflammation and delays type 1 diabetes onset in mice

doi: 10.1016/j.ebiom.2025.105734

Figure Lengend Snippet: TYK2 inhibition reshapes innate and adaptive immunity in blood, spleen, and PLN of RIP-LCMV-GP mice . Peripheral blood mononuclear cells (PBMCs), splenocytes, and immune cells from PLN were isolated and labelled with CD11b + MHCII + CD11c + (dendritic cells, DCs), CD11b + MHCII + F4/80 + (macrophages), CD11b + CD49 + (mature NK cells), and CD11b + CD49 - (immature NK cells). Immune cell characterisation was performed using flow cytometry. (a – c) Percentage of DCs, macrophages, mature NK cells, and immature NK cells from 3, 7, and 14-days post-inoculation from vehicle (grey) and TYK2i-treated (red) RIP-LCMV-GP mice. ( d–f ) Percentage of CD4 + FoxP3 + CD25 + Tregs, CD4 + PD1 + T cells, and CD8 + PD1 + T cells from 3, 7, and 14-days post-inoculation from vehicle (grey) and TYK2i-treated (red) RIP-LCMV-GP mice. Data are presented as mean with 95% CI, and individual data points are included, n = 3–5 mice per condition; Statistical significance was determined by a Mann–Whitney U test.

Article Snippet: Single cell suspensions were permeabilised for intracellular staining using FoxP3 fix/perm buffer (Thermo Fisher Scientific, catalogue #00-5523-00) at 4 °C for 20 min, followed by a wash with permeabilisation buffer.

Techniques: Inhibition, Isolation, Flow Cytometry, MANN-WHITNEY